Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation.
نویسندگان
چکیده
A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores-rhodamine B, a red fluorescent protein, and CdSe quantum dots-while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples.
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ورودعنوان ژورنال:
- Optics express
دوره 18 13 شماره
صفحات -
تاریخ انتشار 2010